ניווט מהיר
פותח על ידי Startup Booster
תחום מחקר: רפואת ילדים וכבד
Synopsis:
My research is focusing mainly in main topics.
1. Liver fibrosis
In my lab we studied the signal transduction pathway of Hepatic Stellate Cells. We demonstrated the antifibrotic effect of Vitamin D on liver fibrosis. We also showed that vitamin D has antiproliferative and antifibrotic effects on liver fibrosis in in vitro, using an in vitro model of primary HSCs (Am J Physiol Gastrointest Liver Physiol, 2015, 308: G112–G120) and in vivo models (Gut, 2011,60-12: 1728-37 . Based on those results vitamin D can be considered as having potential therapeutic value.
2. Milk derived exosomes and their miRNA-Characterization and importance in infant nutrition
Milk is the main nutritional source for newborn mammals and breastfeeding is recognized as one of the most valuable contributors to infant health. Infants that are breastfed enjoy significant health benefits in comparison to those fed with infant formulas (Molecular Nutrition and Food Research,2017, 61(10): 1700009). Recently, our group demonstrated that the skim and fat layer of mammalians milk contains a variety of miRNAs that appear to play important roles in the postnatal development of the offspring. We found high expression of different miRNAs in milk, that may appear to play regulatory roles in many biological and metabolic processes associated with obesity, diabetes, immune system development and diseases prevention. These miRNAs are highly conserved in humans, bovine and goat milk. In contrast, infant formula contains very low amount (almost undetectable) of miRNAs. Furthermore, we showed that exosomes and fat globules present in milk were able to enter into normal and tumor cells and affect their miRNA profile expression and biological functions. For instance (Nutrients 2020;12:2589-2597
מלגות ופרסים
Kamin-Ministry of industry “Additon of exosomes and fat globules isolated from milk into infant formulas”. Golan-Gerstel Regina, Reif S (PI) 980,000 NIS. 2018-2020Israel Science Foundation. The therapeutic effect of milk derived exosomes on colitis. Personal Research Grants Program. Golan-Gerstel Regina, Reif S (PI).870,000 NIS. 2019-2022The Kenneth Rainin Foundation. Milk derived exosomes (MDE) as a therapeutic modality in inflammatory bowel disease (IBD). Abreu M. (co-PI) ,Chowers Y. (co-PI) and Reif S (PI), 300.000USD 2020-2021Chief Scientific Office. "miRNAs in breast milk for premature and mature babies” Reif S, Even Tov S, Reich R. 100,000 NIS 2013Chief Scientific Office. " The role of miRNAs on the anti fibrotic effect of vitamin Din liver fibrosis. Reif S, Weismann Y. 198,000 NIS 2013State of Israel, Ministry of Health ,"The effect of PTHrP on the gut mucosa in health and disease states" Reif S(PI), Bentov A, Weismann Y 2012State of Israel, Ministry of Health, "Izvonot", “The relationship between HIF, PTHrP, HIF, and Vitamin D on liver fibrosis”, Reif S (PI),Abramowitz S, Weisman Y Abramowitz S, Weisman Y - NIS 200,000 2011Anima company – (protein synthesis in HSCs project) Reif S Abramowitz S, Weisman Y – NIS 16,000 2009ISF, “The role of Vitamin D in Liver fibrosis", Reif S(PI), Abramowitz S, Weisman Y – NIS 300,000 2009Tel-Aviv University, Faculty of Medicine: Germanis Alexander grant for Fastroenterology, Reif S (PI) - NIS 30,000 2008Tel Aviv Souraski Medical Center grant for Excellence (for establishing my laboratory) – NIS 320,000 Reif S(PI), Dahan-Bachar L 2007Eldor Memorial Foundation research grant, "The effect of Ras inhibition on colitis induced in animal model”, Oron T(PI), Reif S - NIS 15,000 2004Dani Moran Memorial research grant, Tel-Aviv University, “Matrix Metalloproteinases as markers of inflammation and fibrosis in chronic hepatitis C”, Reif S(PI) – NIS 7,000 2003Alpha One Foundation, USA, “The compound effect of hetrozygocity to α1 antitrypsin deficiency allele on development of liver cirrhosis in CF knock out mice”, Reif S, Brenner D(PI) - $ 100,000 2001Nutritional grant provided by “Tenuva”, research division, Israel, “Effect of low lactose diet on bone mineralization”, Reif S (PI)– NIS 50,000 2000Tel-Aviv University Research Grant (Froindlich and Walenstein), “Extracellular matrix protein expression in neonatal versus adult liver fibrosis”, Reif S (PI)- NIS 30,000 1998NY state University of Buffalo - Calucci Memorial for Liver Study, “Modulation of Extracellular Matrix in Rat Liver During Development”, Reif S (PI)- $ 50,000 1989
פטנטים
Kloog, Y. Chapman, J., Karusis D.M., Bruck R., Reif S., and Brownstein M. Non-malignant disease treatment with Ras antagonists. U.S. patent 6,462,086. 2002Reif S (PI) and Golan-Gerstl R. (PI) “Supplementation of milk formulas with microvesicles isolated from milk”. Hadassit. US 10874114B2 Dec. 29 2020 2017Reif S (PI) and Golan-Gerstl R.(PI) MILK DERIVED EXTRACELLULAR VESICLES FOR USE IN TREATING INFLAMMATORY BOWEL DISEASE. Hadassit PCT submitted in the US No. PCT/IL2020/050518 2019
מאמרים
MiRNA-320a is less expressed and miRNA-148a more expressed in preterm human milk compared to term human milk Yaffa Elbaum Shiff, Shimon Reif, Ronell Marom, Kobi Shiff, Ram Reifen, Regina Milk-derived exosomes (MDEs) have a different biological effect on normal fetal colon epithelial cells compared to colon tumor cells in a miRNA-dependent manner Shimon Reif , Yaffa Elbaum Shiff , Regina Golan-GerstlCharacterization and biological function of milk-derived miRNAs Regina Golan-Gerstl, Yaffa Elbaum Shiff, Vardit Moshayoff, Daniel Schecter, Dena Vitamin D and S-Farnesylthiosalicylic Acid Have a Synergistic Effect on Hepatic Stellate Cells Proliferation Rina Neeman, Shirley Abramovitch, Efrat Sharvit, Galit Elad-Sfadia, Roni Haklai, Yoel Vitamin D and liver fibrosis: let's start soon before it's too late Valerio Nobili, Shimon ReifVitamin D inhibits development of liver fibrosis in an animal model but cannot ameliorate established cirrhosis Shirley Abramovitch, Efrat Sharvit, Yosef Weisman, Amir Bentov, Eli Brazowski, Gili The Ras Antagonist, Farnesylthiosalicylic Acid (FTS), Decreases Fibrosis and Improves Muscle Strength in dy2J/dy2J Mouse Model of Muscular Dystrophy Yoram Nevo, Shlomit Aga-Mizrachi, Edva Elmakayes, Nurit Yanay, Keren Ettinger, Moran Elbaz, Zivia Brunschwig, Oshrat DadAmplified Inhibition of Stellate Cell Activation Pathways by PPAR-γ, RAR and RXR Agonists Efrat Sharvit ,Shirley Abramovitch ,Shimon Reif, Rafael BruckVitamin D inhibits proliferation and profibrotic marker expression in hepatic stellate cells and decreases thioacetamide-induced liver fibrosis in rats Shirley Abramovitch, Liora Dahan-Bachar, Efrat Sharvit, Yosef Weisman, Amir Ben Prevention of Induced Colitis in Mice by the Ras Antagonist Farnesylthiosalicylic Acid Tal Oron, Galit Elad-Sfadia, Roni Haklai, Elizabeta Aizman, Eli Brazowski, Yoel Maternal inflammatory bowel disease has short and long-term effects on the health of their offspring: A multicenter study in Israel Iris Dotan, Arik Alper, Daniel Rachmilewitz, Eran Israeli, Shmuel Odes, Irit Chermesh, Cow and Human Milk-Derived Exosomes Ameliorate Colitis in DSS Murine Model Shimon Reif, Yaffa Elbaum-Shiff, Nickolay Koroukhov, Itamar Shilo, Mirit Musseri, RBreastfeeding and risk of inflammatory bowel disease: a systematic review with meta-analysis Eyal Klement, Regev V Cohen, Jonathan Boxman, Aviva Joseph, Shimon Reif